Visible-microscopic spectroscopy (Vis-MS) is a spectral analysis technique widely used for absorption  and fluorometric  analyses in the analytical chemistry field. The solvent of most samples of VisMS is an aqueous solution, and only a few studies with materials of histological diagnosis  and cytology have been performed. Regarding the staining method used for Vis-MS, studies on Feulgen’s reaction and staining with hematoxylin alone have been performed, but only a few studies have been performed for unstained samples and Papanicolaou staining.