The vaginal wall rugae, wall thickening and lubrication are depending on presence of estrogen. Vagina is estrogen dependent organ as estrogen is responsible for induction of proliferation of vaginal epithelium layers, smooth muscles and collagen fibers so maintains vaginal rugae. Experimental bilateral ovariectomy was done to know the effects of decrease in hormonal levels and their activity in female rats. This experimental ovariectomy has an important role to understand the pathophysiological changes and to help in developments of therapy. So, to improve pathophysiological changes after ovariectomy, estrogen therapy was used as a replacement therapy, but there are many limitations for hormonal uses because its New Possible Approach in Treatment of Experimental Induced Vaginal Atrophy by Bone Marrow-derived Mesenchymal Stem Cells in Treatment of Induced Vaginal Atrophy in Adult Female Albino Rats.