Bone marrow-derived mesenchymal stem cells have the capacity to differentiate into several cell types. Fibroblasts are referred to as mature counterpart of MSCs, even though they can differentiate into follicular dendritic cells and myofibroblasts, the latter type of cells play an important role in wound healing. In addition, fibroblasts contribute to synthesis and remodeling of extracellular matrix (ECM) proteins and are able to respond to external stimuli by production of several cytokines, e.g. CCL2, IL6, and IL 8. Fibroblast-derived cytokines have an impact on the immune system in Graves’ disease and tumor tissue. In this study we show that the fibroblasts exhibit a similar surface protein expression profile when compared to MSC. Furthermore, we demonstrate expression of 4-prolylhydroxylase and fibronectin in both cell types. Microarray analysis of gene expression also revealed many similarities between MSCs and fibroblasts. These data suggest that MSCs and tissue-resident fibroblasts are closely related, if not even representing two variants of the same cell.